Please follow the instruction here, though it talks about visual studio 2010 it should help you to analyze the problem. it may be solved with the steps described there and if not, please let me know...
Why does F10 (step over) in Visual Studio 2010 not work?
Update Adding the answer from the source in case the link will be removed:
In the Options.Keyboard page, please select "Debug.StepOver" from the command listbox, and then put focus to the "Press shortcut keys" textbox and press F10, click Assign button to re-assign shortcut, does it work?
You can also try to run Visual Studio under safe mode, which will
prevents all third-party VSPackages from loading when Visual Studio
starts; if the issue disappear under safe mode, you may consider
checking your installed add-ons or VSPackages.
Second, to log all activity of Visual Studio to a log file for further
troubleshooting, please use the /Log switch, and post the log file
content here, so we can do more investigation on it.
If this feature works well before, and suddenly behave abnormally, it
usually indicate that some files or configurations of Visual Studio
installation is corrupted or missed, you can:
Restores Visual Studio default settings by using "Devenv.exe
/ResetSettings" command. Please backup your settings before restore to
default settings.
Repair/reinstall Visual Studio;
To repair Visual Studio In the Add or Remove Programs dialog box,
select Visual Studio then click Change/Remove.