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I have a function which, given a NIfTI file, generates a set of images. How can I essentially reverse that process with another function which takes in these images and creates a NIfTI file from them?

Scott Caulfield
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  • NIftI file includes more than mere images, Pixel spacing, Affine transform, etc.., please refer to [Nibabel Nifti header](https://nipy.org/nibabel/nifti_images.html#the-nifti-header) – Bilal Aug 01 '23 at 09:55

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You do that with SimpleITK's ImageSeriesReader class. It'd look something like this:

import SimpleITK as sick

reader = sitk.ImageSeriesReader()

reader.SetFileNames(["slice1.png", ..., "sliceN.png"])

volume_image = reader.Execute()

Note that the volume won't have any voxel spacing information, so you might need to call the SetSpacing method

volume_image.SetSpacing([x_spacing, y_spacing, z_spacing])
Dave Chen
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