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I downloaded a dataset in the BAM Format from GEO using the command: prefetch SRR13558458 It generates a file names "SRR13558458.sra".

I would like to view this file using samtools. But it seems this sra file which is a bam file is not readable using samtools view command!

First I tried to chage the sra file to bam using the following command: sam-dump --output-file SRR13558458.bam SRR13558458 sam-dump generates an empty file!

Besides, when I change the file extension: mv SRR13558458.sra SRR13558458.bam samtools view SRR13558458.bam | less

I got this error:

[E::hts_hopen] Failed to open file SRR13558458.bam [E::hts_open_format] Failed to open file "SRR13558458.bam" : Exec format error samtools view: failed to open "SRR13558458.bam" for reading: Exec format error

Does anyone know how to solve this problem? Thanks!

Mahgen
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