I generated a heatmap with ggplot, and order the samples by using hclust, However, I still need more reordering to get all the similar values corespondent with one of the samples in the ordered cluster. Here I generate a samples data to explain better.
set.seed(99)
M <- data.frame(names = paste0("g", seq(1,30)), S1 = runif(30, 0 , 8), S2 = runif(30, -4, 5), S3 = runif(30, -5, 5))
M.mat <- M %>%
tibble::column_to_rownames('names') %>%
as.matrix()
M.dendro <- as.dendrogram(hclust(d = dist(x = M.mat)))
dendro.plot <- ggdendrogram(data = M.dendro, rotate = TRUE) +
theme(axis.text.y = element_text(size = 6))
print(dendro.plot)
str(M.dendro)
dend.order <- order.dendrogram(M.dendro)
df <- melt(M, id.vars = "names")
df$names <- factor(x = df$names,
levels = M$names[dend.order],
ordered = TRUE)
ggplot(df, aes(x = names, y = variable, fill = value)) +
geom_tile(color = "black") +
scale_fill_gradient2(low = muted("steelblue"), mid = "white", high = muted("red3"),
midpoint = 0, space = "Lab", na.value = "grey50",
guide = "colourbar", aesthetics = "fill"
) +
theme(axis.text.x = element_text(angle = 90, hjust=1), legend.key.size = unit(0.4, "cm")) +
coord_fixed()
For the generated heatmap, I need reorder it such that all the dark blue be on the bottom, the middle color and then the red on the top based on samples S3. Thank you 
