Yes, you will have longer cold starts, resulting in much higher response times.
It is really dependent on the image you're downloading from ECR but in general, it will be slower as a Docker container is being used instead of Lambda managing the runtime environment for you (which reduces the time to it takes to start a new execution environment for cold Lambdas).
The main cause is the size of the ECR image, which is also why there is a limit on large Lambda ZIP archives.
You can see here how the size will affect the 2 tasks that run during cold start, defined by AWS as Download your code & Start new execution environment.

I would advise you to definitely use the managed runtime as opposed to containers unless you need to use them as it will automatically result in faster execution.