Networking in nodejs does not use the threadpool so switching that will not affect your network I/O throughput. Networking uses OS APIs that are already asynchronous and non-blocking.
Your Javascript that runs when an incoming request is processed does not use the thread pool either.
Disk I/O does use the threadpool, but if you're only accessing one physical disk drive, then you may not benefit much from making the threadpool larger because there's only one physical disk servo that can only be in one place at a time anyway so running 20 disk requests in parallel doesn't necessarily help you if they are all competing for the same disk head positioning. In fact, it might even make things worse as the OS tries to timeslice between all the different threads causing the disk head to move around more than optimal to serve each thread.
To serve 1000 requests per second, you will have to benchmark and test and find out where your bottlenecks are. If I had to guess I'd bet the bottleneck would be your database in which case reconfiguring nodejs settings isn't where you need to concentrate your effort. But, in any case, only once you've determined where the bottleneck is in your particular application can you properly figure out what options might help you with that bottleneck. Also, keep in mind that serving 1000 requests/sec means you can't be running Javascript per request that takes more than 1ms for each request. So, you will probably have to cluster your server too (typically one cluster per physical CPU core in your server hardware). So, if you have an 8-core server, you would set up an 8-node cluster.
For example if you are CPU limited in your nodejs process with the running of your own Javascript, then perhaps you want to implement nodejs clustering to get multiple CPUs all running different requests. But, if the real bottleneck is in your database, then cluster your nodejs request handlers won't help with the database bottleneck.
Benchmark, measure, come up with theories based on the data for what to change, design specific tests to measure that, then implement one of the theories and measure. Adjust based on what you measure. You can only really do this properly (without wasting a lot of time in unproductive directions) by measuring first, making appropriate adjustments and then measuring progress.