Thanks for your responding.
I have corrected my typo and changed my configuration files as innodb_thread_concurrency = 0 as you mentioned.
I tried to configure very low-performing-consuming parameters and I have read about that innodb_file_per_table parameters could effect the RAM usage, so I decided to comment it out. Actually I couldn't figure out what this parameters use for clearly.
Let me tell you my some observation after I have restarted both DB servers.
First of all I stopped all application that making connection to db-1 (Actually we are making all db connections through DB1), then restarted both DB-servers. First of all, let me clear that there was no any traffic when I started applications.
After I start all appcalitons that we use, I realised that I was seeing about 120 processes that created by our user in PROCESSLIST in the INFORMATION_SCHEMA. Also I know that some of these connections don't make anything but using connnection_pool.
I was set up 100s wait_timeout, after 100s later, PROCESSLIST down to "27" and threads_cache became 92.
I also know that these 27 connection was used by some applications that running some check queries in spesific time interval. (10sec, 4sec, 2sec) So when they run queries , their connection_time return 0 and count again as they are never closed.
I believe that in the peak_time, we are gonna need thread_cache_size, so configuring this parameter as 256 would be good for our system.

I know it is so hard to understand for now but can you please check my innodb engine status and give me some advice as I am not very good at concept of database management systems.
=====================================
2020-05-08 01:10:28 0x7fd8cc55c700 INNODB MONITOR OUTPUT
=====================================
Per second averages calculated from the last 7 seconds
-----------------
BACKGROUND THREAD
-----------------
srv_master_thread loops: 4897 srv_active, 0 srv_shutdown, 371 srv_idle
srv_master_thread log flush and writes: 5266
----------
SEMAPHORES
----------
OS WAIT ARRAY INFO: reservation count 34869
OS WAIT ARRAY INFO: signal count 121009
RW-shared spins 0, rounds 214984, OS waits 15858
RW-excl spins 0, rounds 1540716, OS waits 11401
RW-sx spins 52309, rounds 782230, OS waits 3893
Spin rounds per wait: 214984.00 RW-shared, 1540716.00 RW-excl, 14.95 RW-sx
------------
TRANSACTIONS
------------
Trx id counter 211830460
Purge done for trx's n:o < 211830450 undo n:o < 0 state: running but idle
History list length 1
LIST OF TRANSACTIONS FOR EACH SESSION:
---TRANSACTION 422083415170480, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415166264, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415162048, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415157832, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415153616, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415149400, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415145184, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415140968, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415136752, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415132536, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415128320, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415124104, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415119888, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415115672, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415111456, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415107240, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415103024, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415098808, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415094592, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415090376, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415086160, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415081944, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415077728, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415073512, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415069296, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415065080, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415060864, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415056648, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
---TRANSACTION 422083415052432, not started
0 lock struct(s), heap size 1136, 0 row lock(s)
--------
FILE I/O
--------
I/O thread 0 state: waiting for completed aio requests (insert buffer thread)
I/O thread 1 state: waiting for completed aio requests (log thread)
I/O thread 2 state: waiting for completed aio requests (read thread)
I/O thread 3 state: waiting for completed aio requests (read thread)
I/O thread 4 state: waiting for completed aio requests (read thread)
I/O thread 5 state: waiting for completed aio requests (read thread)
I/O thread 6 state: waiting for completed aio requests (read thread)
I/O thread 7 state: waiting for completed aio requests (read thread)
I/O thread 8 state: waiting for completed aio requests (read thread)
I/O thread 9 state: waiting for completed aio requests (read thread)
I/O thread 10 state: waiting for completed aio requests (write thread)
I/O thread 11 state: waiting for completed aio requests (write thread)
I/O thread 12 state: waiting for completed aio requests (write thread)
I/O thread 13 state: waiting for completed aio requests (write thread)
I/O thread 14 state: waiting for completed aio requests (write thread)
I/O thread 15 state: waiting for completed aio requests (write thread)
I/O thread 16 state: waiting for completed aio requests (write thread)
I/O thread 17 state: waiting for completed aio requests (write thread)
Pending normal aio reads: [0, 0, 0, 0, 0, 0, 0, 0] , aio writes: [0, 0, 0, 0, 0, 0, 0, 0] ,
ibuf aio reads:, log i/o's:, sync i/o's:
Pending flushes (fsync) log: 0; buffer pool: 0
363895 OS file reads, 105906 OS file writes, 18722 OS fsyncs
0.00 reads/s, 0 avg bytes/read, 6.14 writes/s, 2.43 fsyncs/s
-------------------------------------
INSERT BUFFER AND ADAPTIVE HASH INDEX
-------------------------------------
Ibuf: size 1, free list len 13430, seg size 13432, 2410 merges
merged operations:
insert 72630, delete mark 0, delete 0
discarded operations:
insert 0, delete mark 0, delete 0
Hash table size 6374293, node heap has 108 buffer(s)
Hash table size 6374293, node heap has 64 buffer(s)
Hash table size 6374293, node heap has 64 buffer(s)
Hash table size 6374293, node heap has 18 buffer(s)
Hash table size 6374293, node heap has 10 buffer(s)
Hash table size 6374293, node heap has 72 buffer(s)
Hash table size 6374293, node heap has 4 buffer(s)
Hash table size 6374293, node heap has 634 buffer(s)
3473.22 hash searches/s, 553.49 non-hash searches/s
---
LOG
---
Log sequence number 129572121784
Log flushed up to 129572121784
Pages flushed up to 129572121784
Last checkpoint at 129572120321
0 pending log flushes, 0 pending chkp writes
3127 log i/o's done, 0.71 log i/o's/second
----------------------
BUFFER POOL AND MEMORY
----------------------
Total large memory allocated 26398949376
Dictionary memory allocated 278784
Buffer pool size 1572672
Free buffers 1200805
Database pages 370893
Old database pages 137029
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 2349, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 363786, created 7107, written 94745
0.00 reads/s, 0.00 creates/s, 4.43 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 370893, unzip_LRU len: 0
I/O sum[0]:cur[24], unzip sum[0]:cur[0]
----------------------
INDIVIDUAL BUFFER POOL INFO
----------------------
---BUFFER POOL 0
Buffer pool size 262112
Free buffers 198770
Database pages 63178
Old database pages 23341
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 372, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 62294, created 884, written 22682
0.00 reads/s, 0.00 creates/s, 2.14 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 63178, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
---BUFFER POOL 1
Buffer pool size 262112
Free buffers 198593
Database pages 63353
Old database pages 23406
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 365, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 62005, created 1348, written 17745
0.00 reads/s, 0.00 creates/s, 0.86 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 63353, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
---BUFFER POOL 2
Buffer pool size 262112
Free buffers 202987
Database pages 58961
Old database pages 21784
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 388, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 58042, created 919, written 15919
0.00 reads/s, 0.00 creates/s, 1.00 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 58961, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
---BUFFER POOL 3
Buffer pool size 262112
Free buffers 199895
Database pages 62058
Old database pages 22928
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 439, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 60791, created 1267, written 12163
0.00 reads/s, 0.00 creates/s, 0.00 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 62058, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
---BUFFER POOL 4
Buffer pool size 262112
Free buffers 201239
Database pages 60712
Old database pages 22431
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 382, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 59190, created 1522, written 12539
0.00 reads/s, 0.00 creates/s, 0.43 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 60712, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
---BUFFER POOL 5
Buffer pool size 262112
Free buffers 199321
Database pages 62631
Old database pages 23139
Modified db pages 0
Percent of dirty pages(LRU & free pages): 0.000
Max dirty pages percent: 80.000
Pending reads 0
Pending writes: LRU 0, flush list 0, single page 0
Pages made young 403, not young 0
0.00 youngs/s, 0.00 non-youngs/s
Pages read 61464, created 1167, written 13697
0.00 reads/s, 0.00 creates/s, 0.00 writes/s
Buffer pool hit rate 1000 / 1000, young-making rate 0 / 1000 not 0 / 1000
Pages read ahead 0.00/s, evicted without access 0.00/s, Random read ahead 0.00/s
LRU len: 62631, unzip_LRU len: 0
I/O sum[0]:cur[4], unzip sum[0]:cur[0]
--------------
ROW OPERATIONS
--------------
0 queries inside InnoDB, 0 queries in queue
0 read views open inside InnoDB
Process ID=31986, Main thread ID=140569828304640, state: sleeping
Number of rows inserted 276741, updated 1143, deleted 276686, read 179703655
0.00 inserts/s, 0.29 updates/s, 0.00 deletes/s, 109971.15 reads/s
Number of system rows inserted 0, updated 0, deleted 0, read 0
0.00 inserts/s, 0.00 updates/s, 0.00 deletes/s, 0.00 reads/s
----------------------------
END OF INNODB MONITOR OUTPUT
============================
I will share threads status and INNODB ENGINE STATUS after 1-2 day later, too.
I hope we won't have face with any lock wait timeout issues.
Have a nice day.