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I am new to QIIME. By following the Illumina overview tutorial, I ran:

!validate_mapping_file.py -o vmf-map/ -m map.tsv

and then I got an error message:

/bin/sh: validate_mapping_file.py: command not found

I also try to run command

!qiime, !MacQIIME and !QIIME, but my computer also cannot find those commands.

I have installed the QIIME, the command:

source activated qiime

Works well

Could anyone help me?

Thanks!

Rafael Marques
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  • Do you see the file validate_mapping_file.py in your working directory or in your path? If yes try to make it executable: ```chmod +x validate_mapping_file.py``` or call it via python: ```python validate_mapping_file.py -o vmf-map/ -m map.tsv```. If you cannot find the script something went wrong with your installation. – flappix Feb 02 '20 at 00:10
  • Where do you enter these commands? Did you mean `python validate_mapping_file.py -o vmf-map/ ...`? – mkrieger1 Feb 02 '20 at 00:10

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