I have an image that I want to detect bright spots. I can detect particles according to the characteristics I want. but when linking these particles I found, I can't eliminate unwanted particles. The line of code that detects the particles is as follows.
f = tp.batch(frame_list[:],7, invert = False,
minmass = 15, maxsize = 17, preprocess = False)
t = tp.link_df(f,3, memory =5)
there are particles that are detected only in 1 image and then not visible. I don't want them.Shouldn't the 2nd parameter in t variable do this job? even if she doesn't, I can loop it and find it again.but this will force the program. Because we have 550 thousand lines yield.How can I deal with this? I checked the library, but nothing else