I first upload the table. The table contains 9 rows, 6 of them are factors and the 3 left are discrete measures of growth rate of 152 individuals (n01,n02,n03). Then I specify the factors:
`r$feed <- factor (r$feed)`
`r$ph <- factor (r$ph)`
`r$aq <- factor (r$aq)`
`r$ind <- factor (r$ind)`
`r$wc <- factor (r$wc)`
`r$p0<- factor (r$p0)`
Following, I perform I melt the dataframe into a new table "r2" with the factors I am interested in and remove NA values with na.omit function.
`r2 <- data.table::melt(r,id.vars=c("feed","ph","aq","wc"),
measure=c("n01","n12","n23"),
variable.name="time",value.name="G")`
`r2<-na.omit(r2)`
r2 looks like this:
data.frame(
G = c(0.184, 0.087, 1.747, 0.11, 0.39, 0.062, 0.08, 0.189, 0.068,
0.262, 0.048, 0.029, 0, 0.229, 0.175),
feed = as.factor(c("HF", "HF", "HF", "HF", "HF", "HF", "HF", "HF",
"HF", "HF", "HF", "HF", "HF", "HF", "HF")),
ph = as.factor(c("8.1", "8.1", "8.1", "8.1", "8.1", "8.1", "8.1",
"8.1", "8.1", "8.1", "8.1", "8.1", "8.1", "8.1",
"8.1")),
aq = as.factor(c("1", "1", "1", "1", "1", "1", "2", "2", "2", "2",
"2", "2", "2", "3", "3")),
wc = as.factor(c("3", "3", "2", "3", "2", "4", "3", "4", "2", "2",
"3", "3", "1", "4", "3")),
time = as.factor(c("n01", "n01", "n01", "n01", "n01", "n01", "n01",
"n01", "n01", "n01", "n01", "n01", "n01", "n01",
"n01"))
)
After that, I set the fixed variance and apply and perfom 2 gls models, like this:
`vfix3 <- varIdent(form=~1|time*factor(aq))
mix1 <- gls(G ~ ph+feed, weights=vfix3,data=r2)
mix3 <- gls(G ~ ph+feed+wc+time, weights=vfix3,data=r2)`
The models seem to work fine since I can get the summary and anova of them. Then, I try to run post-hoc pairwise comparisons with lsmeans function from the package emmeans as it follows:
print(lsmeans(mix1, list(pairwise~ph|feed), adjust="tukey"))
lsmeans seems to work fine with 2-factor model mix1. However, when executing lsmeans on model mix3 this error pops up:
Error in crossprod(x, y) : requires numeric/complex matrix/vector arguments
I have tried to transform the model to a matrix, but for the lsmeans
function it is not a correct object. I have also tried not setting the factors and leaving the columns as numeric, but same error pops up. When reading about lsmeans function there I can't find any crossprod function related to it.