I have a disk that I want to cut it in wedges, just like a pizza, I did this with ImageJ,
but as you can see in the picture, there are some pixels that has a fraction inside a ROI and the other fraction inside the other ROI.
Is there a way to find how much fraction of the area of the pixel lies in either ROIs? Ideally, I wouldn't want to do any kind of Interpolation, and write a python code for automating that.
Eventually, I'd want to measure the raw intensity of each ROI in each time frame and generate a kymograph of the resulting array.
Any kind of help would be much appreciated, even if it doesn't answer my question directly.