When running the client daemon you can add the 'connect' parameter to specify the IP:Port of the computer you want to try to connect to when starting.
bitcoind --daemon -connect=127.0.0.1:9090
This usually has to be done when the ip's of nodes hard coded into your blockchain codebase have not been updated to the IP's where you have nodes running. Thus peers.dat does not know where to look for a connection to the blockchain.
You can also specify these in the config file with the addnode parameter. e.g.
server=1
rpcport=9090
rpcuser=bitcoinrpc
rpcpassword=3QtnxrB7P5y4EpBdad1MkCeB2RHmArvcarw7udgXsAce
addnode=10.0.0.2:8333