You can try to create a UIView to display the AVPlayerLayer and set its position to layout your AVPlayer. We can either put this contentView in StackView or outside the StackView in the ViewController's View.
I recommend to put this view directly in the ViewController's View so that we can use autolayout to layout it.
Firstly, I create a UIView whose top constraint equal to your Vertical StackView's bottom constraint and bottom constraint equal to your horizontal StackView's top constraint like:
UIView contentView = new UIView();
contentView.TranslatesAutoresizingMaskIntoConstraints = false;
View.AddSubview(contentView);
NSLayoutConstraint topConstraint = NSLayoutConstraint.Create(contentView, NSLayoutAttribute.Top, NSLayoutRelation.Equal, VerticalStackView, NSLayoutAttribute.Bottom, 1, 0);
NSLayoutConstraint leadConstraint = NSLayoutConstraint.Create(contentView, NSLayoutAttribute.Leading, NSLayoutRelation.Equal, View, NSLayoutAttribute.Leading, 1, 0);
NSLayoutConstraint trailConstraint = NSLayoutConstraint.Create(contentView, NSLayoutAttribute.Trailing, NSLayoutRelation.Equal, View, NSLayoutAttribute.Trailing, 1, 0);
NSLayoutConstraint heightConstraint = NSLayoutConstraint.Create(contentView, NSLayoutAttribute.Height, NSLayoutRelation.Equal, null, NSLayoutAttribute.NoAttribute, 0, 100);
NSLayoutConstraint bottomConstraint = NSLayoutConstraint.Create(contentView, NSLayoutAttribute.Bottom, NSLayoutRelation.Equal, HorizontalStackView, NSLayoutAttribute.Top, 1, 0);
View.AddConstraints(new NSLayoutConstraint[] { topConstraint, leadConstraint, trailConstraint, bottomConstraint });
contentView.AddConstraint(heightConstraint);
Then you can put your AVPlayerLayer in this view and let its frame equal to this view like:
AVPlayerItem playerItem = new AVPlayerItem(new NSUrl(NSBundle.MainBundle.PathForResource("test.mp4", null), false));
aVPlayer = new AVPlayer(playerItem);
AVPlayerLayer playerLayer = AVPlayerLayer.FromPlayer(aVPlayer);
contentView.Layer.AddSublayer(playerLayer);
//update its frame immediately
contentView.LayoutIfNeeded();
playerLayer.Frame = contentView.Bounds;
If you do want to achieve this in StackView. Just put the contentView in the StackView and set its height constraint to show the AVPlayer.