While you can use a 4D matrix to apply multiple transformations in one step, for the most part I would say it is more common to access each property either as an array of three or four values or to directly access each element.
import bpy
obj = bpy.context.active_object
obj.location.x += 1.0
obj.location.y -= 0.2
obj.location.z += 0.8
obj.rotation_euler = (radians(45.0),radians(15.8), radians(0.0))
Blender includes a full python interpreter, so you should be able to do most of what you want using blender without having to export any data.
If you want to try blender, you can get more blender specific help at blender.stackexchange.