Is IE6 the only browser that doesn't support PNG transparency?
4 Answers
From: http://www.alistapart.com/articles/pngopacity/
It turns out that most of the latest versions of the major browsers fully support alpha transparency with PNG – namely, Netscape 6, Opera 6, and recently-released Mozilla 1, all on Windows; and, for the Mac, Internet Explorer 5, Netscape 6, Opera 5, Mozilla 1, OmniWeb 3.1, and ICab 1.9. Incredibly, PNG even works on Opera 6 for Linux, on WebTV, and on Sega Dreamcast.
IE5.5+/Win, bless its heart, will, in fact, display a PNG, but it doesn’t natively support alpha transparency. In IE5.5+/Win, the transparent area of your PNG will display at 100% opacity – that is, it won’t be transparent at all.

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Pretty sure that IE5 doesn't either.

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1I meant on browsers that are higher than IE6 ^_^ – Ben Jan 13 '11 at 15:04
I think it's the only commonly used browser that doesn't support PNG transparency.
Obviously other older browsers such as Netscape 3, IE5 et al also lack support for this but I'm assuming your question is about browsers in every day use.

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