You will need to install mingw32-make. This is a
Windows of port of GNU Make,
a software-build tool that is supported on all operating systems,
indeed the daddy of such tools.
But make alone will not suffice. To build primer3 you will
need a Windows port of the whole GNU toolchain for building software
from source code. Without that, running make by itself will
just expose the absence of the GCC compiler and linker that it
expects to do its bidding.
This is quite a lot of software, but it is easy and quick to install and there
are several open-source offerings. I suggest you go to TDM GCC
and download the TDM64 bundle. This will give you an executable installer.
Just run it and you will end up with the complete GNU toolchain, including,
mingw32-make, in your chosen installation directory.
It will also install in your Windows launch menu the MinGW command prompt.
Launch this and you will be presented with a Windows commandline console
with its environment set up to find and run any of the GNU tools.
In this console change directory to your primer3-X.Y.Z/test directory
and then run mingw32-make TESTOPTS=--windows as per documentation.
Be forwarned that the self-tests of primer3 that are executed to
verify the build may take 1/2 hr. to 1 hr. to run, depending on your
hardware, but they will finish successfully with the steps I've
described, barring problems specific to your machine. It is a foolproof-simple build.
All the built executables are deposited in the primer3-X.Y.Z/src
directory. You may want to move them somewhere more convenient
in in your PATH.
It does seem oddly amateurish that the documentation simply
directs you to run mingw32-make with no preliminary account of
what that is or how to install it, while on the other hand it
advises that you must install perl and strongly recommends a
specific perl distribution; but evidently primer3 is open-source
scientfic software and its documentation is not bad by the standard
of that genre.
