I'm running quite a complex code so I won't bother with details as I've had it working before but now im getting this error.
Particle is a 3D tuple filled with 0 or 255, and I am using the scipy centre of mass function and then trying to turn the value into its closest integer (as I'm dealing with arrays). The error is found with on the last line... can anyone explain why this might be??
2nd line fills Particle 3rd line deletes any surrounding particles with a different label (This is in a for loop for all labels)
Particle = []
Particle = big_labelled_stack[x_start+20:x_stop+20,y_start+20:y_stop+20,z_start+20:z_stop+20]
Particle = np.where(Particle == i ,255,0)
CoM = scipy.ndimage.measurements.center_of_mass(Particle)
CoM = [ (int(round(x)) for x in CoM ]
Thanks in advance. If you need more code just ask but I dont think it will help you and its very messy.
################## MORE CODE border = 30
[labelled_stack,no_of_label] = label(labelled,structure_array,output_type)
# RE-LABEL particles now no. of seeds has been reduced! LAST LABELLING
#Increase size of stack by increasing borders and equal them to 0; to allow us to cut out particles into cube shape which else might lye outside the border
h,w,l = labelled.shape
big_labelled_stack = np.zeros(shape=(h+60,w+60,l+60),dtype=np.uint32)
# Creates an empty border around labelled_stack full of zeros of size border
if (no_of_label > 0): #Small sample may return no particles.. so this stage not neccesary
info = np.zeros(shape=(no_of_label,19)) #Creates array to store coordinates of particles
for i in np.arange(1,no_of_label,1):
coordinates = find_objects(labelled_stack == i)[0] #Find coordinates of label i.
x_start = int(coordinates[0].start)
x_stop = int(coordinates[0].stop)
y_start = int(coordinates[1].start)
y_stop = int(coordinates[1].stop)
z_start = int(coordinates[2].start)
z_stop = int(coordinates[2].stop)
dx = (x_stop - x_start)
dy = (y_stop - y_start)
dz = (z_stop - z_start)
Particle = np.zeros(shape=(dy,dx,dz),dtype = np.uint16)
Particle = big_labelled_stack[x_start+30:x_start+dx+30,y_start+30:y_start+dy+30,z_start+30:z_start+dz+30]
Particle = np.where(Particle == i ,255,0)
big_labelled_stack[border:h+border,border:w+border,border:l+border] = labelled_stack
big_labelled_stack = np.where(big_labelled_stack == i , 255,0)
CoM_big_stack = scipy.ndimage.measurements.center_of_mass(big_labelled_stack)
C = np.asarray(CoM_big_stack) - border
if dx > dy:
b = dx
else: #Finds the largest of delta_x,y,z and saves as b, so that we create 'Cubic_Particle' of size 2bx2bx2b (cubic box)
b = dy
if dz > b:
b = dz
CoM = scipy.ndimage.measurements.center_of_mass(Particle)
CoM = [ (int(round(x))) for x in CoM ]
Cubic_Particle = np.zeros(shape=(2*b,2*b,2*b))
Cubic_Particle[(b-CoM[0]):(b+dx-CoM[0]),(b-CoM[1]):(b+dy-CoM[1]),(b-CoM[2]):(b+dz-CoM[2])] = Particle
volume = Cubic_Particle.size # Gives volume of the box in voxels
info[i-1,:] = [C[0],C[1],C[2],i,C[0]-b,C[1]-b,C[2]-b,C[0]+b,C[1]+b,C[2]+b,volume,0,0,0,0,0,0,0,0] # Fills an array with label.No., size of box, and co-ords
else:
print('No particles found, try increasing the sample size')
info = []
Ok, so I have a stack full of labelled particles, there are two things I am trying to do, first find the centre of masses of each particle with respect ot the labelled_stack which is what CoM_big_labelled_stack (and C) does. and stores the co-ords in a list (tuple) called info. I am also trying to create a cubic box around the particle, with its centre of mass as the centre (which is relating to the CoM variable), so first I use the find objects function in scipy to find a particle, i then use these coordinates to create a non-cubic box around the particle, and find its centre of mass.I then find the longest dimension of the box and call it b, creating a cubic box of size 2b and filling it with particle in the right position.
Sorry this code is a mess, I am very new to Python
