Is there any free and open-source option to run python from an usb-stick on Windows system (i.e. python installed on an usb-stick ) and can run on any Windows system just by pluging-in the usb-stick?
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Try Portable Python.

Vlad the Impala
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Can i install on it different packages if needed ? – oren Mar 17 '10 at 20:32
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3Python on a stick, sounds delicious – Morlock Mar 17 '10 at 20:33
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1@oren: yes, that should be fine. – Vlad the Impala Mar 17 '10 at 20:38
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1This answer is obsolete. Portable Python site now says "Portable Python is not being developed anymore" and there is no download links, just links to other python distributions. – Marko Kohtala Sep 08 '15 at 09:06