I want to remove the skin particles from my X-Ray of hand image. I want bones as white Color and all other areas including the skin area in the x ray as black. I am doing my project in mat lab.
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You can use this script for Local Adaptive Thresholding.
Or if you want bones as white color you should use sobel-operator, as in this example.

Plo_Koon
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I tried that methods but the bone seperation is difficult and the some of the bone regions are also removing by this methode.. – Nikhil Dharman Nov 16 '13 at 15:23
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A simple way,you can just change the value of a pixel, when it is higher than 125 change it to 255,or set 0. the number 125,you can change it,to get a best one. you can search "binarization" on google to get more information.

zawdd
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But i want to separate each bones in an x ray also. that means each bone white colour and all others background and skin and gap between each bones are also to be black.. can you help? – Nikhil Dharman Nov 16 '13 at 15:22