So I have Windows 2003 with IIS6 Hosting an MVC web app that requires domain authentication. Any Good Ideas on how to do this. The errors I get is 400, 401 or service unavailable. I have followed the guides on setting up MVC Isapi however in doing wireshark monitoring I see that the domain responds but the local server denies the authentication. I have gone by this technet article. http://www.microsoft.com/technet/prodtechnol/WindowsServer2003/Library/IIS/1f708fa2-54ab-4da0-a5eb-d6a6c453c939.mspx?mfr=true However this does not work. Help.
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You should try as following:
Go to Properties of your website. Go to Directory Security Enable anonymours User and there you will be able to enter Domain User and Password.
Then,
Go to command prompt and register IIS as below, this is required for Service Unavailable Error
cd \ ( It will take you to C:) cd \Windows\Microsoft.NET\Framework\V2.0.50727
aspnet_regiis -e aspnet_regiis -i reset iis.

Ankur Dholakiya
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This line crashes or will not run. aspnet_regiis -e aspnet_regiis -i reset iis – mikedopp Dec 08 '10 at 19:29
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Trying this. http://support.microsoft.com/kb/326020 – mikedopp Dec 08 '10 at 21:59