The error message you're describing shows the ASA's stateful packet inspection at play. The "no connection" means just that: the ASA doesn't have an entry in its connection table for a TCP connection between 192.168.251.2:4283 and 192.168.253.16:3389.
I would set up a capture as follows:
asa(config)# access-list RDPcap permit tcp host 192.168.251.2 host 192.168.253.16 eq 3389
asa(config)# access-list RDPcap permit tcp host 192.168.251.2 host 192.168.253.17 eq 3389
asa(config)# access-list RDPcap permit tcp host 192.168.251.2 host 192.168.253.20 eq 3389
asa(config)# access-list RDPcap permit tcp host 192.168.253.16 eq 3389 host 192.168.251.2
asa(config)# access-list RDPcap permit tcp host 192.168.253.17 eq 3389 host 192.168.251.2
asa(config)# access-list RDPcap permit tcp host 192.168.253.20 eq 3389 host 192.168.251.2
asa# capture RDPcap1 access-list RDPcap interface LAN251
asa# capture RDPcap2 access-list RDPcap interface LAN253
where LAN251 is the interface connected to the 192.168.251.0/24 network and LAN253 is the interface connected to the 192.168.253.0/24 network.
Then try connecting via RDP and see what's in the captures.
My bet is that you'll find that when you're trying to connect to one of the IPs (you'll see a SYN packet sent from a random port >1024 on 192.168.251.2 to port 3389 on 192.168.253.16/17/20), you'll see the response (SYN-ACK) come back from a different IP address, and the firewall will receive that SYN-ACK packet but will not forward it on to your RDP client.
If this is the case, the solution is not on the ASA. You need to figure out how to set up whatever clustering or load-balancing platform you're using to work correctly with RDP.
If this is not the case, please paste the captures so the community can analyze them.
