U.S. Route 301

U.S. Route 301 (US 301) is a spur of U.S. Route 1 running through the South Atlantic States. It runs 1,099 miles (1,769 km) from Biddles Corner, Delaware, at Delaware Route 1 to Sarasota, Florida, at U.S. Route 41. It passes through the states of Delaware, Maryland, Virginia, North Carolina, South Carolina, Georgia, and Florida. It goes through the cities of Middletown, Delaware; Annapolis, Maryland; Richmond and Petersburg, Virginia; Rocky Mount, Wilson, and Fayetteville, North Carolina; Florence, South Carolina; Statesboro and Jesup, Georgia; and Ocala, Zephyrhills, and Sarasota, Florida.

US 301 parallels Interstate 95 for much of its routing through The Carolinas and Virginia including short concurrencies in Santee, South Carolina, and Lumberton, North Carolina. It has a number of other concurrencies along its route.

Originally, US 301 was much shorter. Its former northern terminus was in Baltimore, Maryland. It followed the alignment of the current Maryland Route 3, portions of the Baltimore Beltway, and Maryland Route 648. US 301 ended in Southwestern Baltimore on Monroe Street at the intersection with US 1. (Later, MD 3 was supplanted north of Millersville by Interstate 97.) It was later extended into the state of Delaware, along a different alignment that bypassed Baltimore altogether. Until 2019, the northern terminus was at US 40 in Glasgow, Delaware; the state of Delaware built a new tolled freeway to carry US 301, and it terminates southeast of the former terminus, at Delaware Route 1 just south of the Chesapeake & Delaware Canal. The original southern end was in Summerton, South Carolina, and it has been extended south many times, now to US 41 in Sarasota, Florida.