Trichrome staining

Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Staining differentiates tissues by tinting them in contrasting colours. It increases the contrast of microscopic features in cells and tissues, which makes them easier to see when viewed through a microscope.

"Trichrome" redirects here. For three color vision, see Trichromacy. For the band and album, see Trichromes. For three-color systems, see CMY color model, RGB color model, and RYB color model. Not to be confused with Trichome.

The word trichrome means "three colours". The first staining protocol that was described as "trichrome" was Mallory's trichrome stain, which differentially stained erythrocytes to a red colour, muscle tissue to a red colour, and collagen to a blue colour. Some other trichrome staining protocols are the Masson's trichrome stain, Lillie's trichrome, and the Gömöri trichrome stain.

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