DNA polymerase II

DNA polymerase II (also known as DNA Pol II or Pol II) is a prokaryotic DNA-dependent DNA polymerase encoded by the PolB gene.

This article is about the DNA polymerase. For the RNA polymerase, see RNA polymerase II.
DNA Polymerase II
Crystal structure of DNA pol II (based on PDB entry 3K5M)
Identifiers
OrganismEscherichia coli
(str. K-12 substr. MG1655)
SymbolpolB
Entrez944779
PDB3K5M
RefSeq (Prot)NP_414602.1
UniProtP21189
Other data
EC number2.7.7.7
Chromosomegenome: 0.06 - 0.07 Mb
Search for
StructuresSwiss-model
DomainsInterPro

DNA Polymerase II is an 89.9-kDa protein and is a member of the B family of DNA polymerases. It was originally isolated by Thomas Kornberg in 1970, and characterized over the next few years. The in vivo functionality of Pol II is under debate, yet consensus shows that Pol II is primarily involved as a backup enzyme in prokaryotic DNA replication. The enzyme has 5′→3′ DNA synthesis capability as well as 3′→5′ exonuclease proofreading activity. DNA Pol II interacts with multiple binding partners common with DNA Pol III in order to enhance its fidelity and processivity.

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