Phosphoglycerate kinase
Phosphoglycerate kinase (EC 2.7.2.3) (PGK 1) is an enzyme that catalyzes the reversible transfer of a phosphate group from 1,3-bisphosphoglycerate (1,3-BPG) to ADP producing 3-phosphoglycerate (3-PG) and ATP :
- 1,3-bisphosphoglycerate + ADP ⇌ glycerate 3-phosphate + ATP
| Phosphoglycerate kinase | |||||||||
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| Identifiers | |||||||||
| EC no. | 2.7.2.3 | ||||||||
| CAS no. | 9001-83-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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| Phosphoglycerate kinase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Structure of yeast phosphoglycerate kinase. | |||||||||
| Identifiers | |||||||||
| Symbol | PGK | ||||||||
| Pfam | PF00162 | ||||||||
| InterPro | IPR001576 | ||||||||
| PROSITE | PDOC00102 | ||||||||
| SCOP2 | 3pgk / SCOPe / SUPFAM | ||||||||
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Like all kinases it is a transferase. PGK is a major enzyme used in glycolysis, in the first ATP-generating step of the glycolytic pathway. In gluconeogenesis, the reaction catalyzed by PGK proceeds in the opposite direction, generating ADP and 1,3-BPG.
In humans, two isozymes of PGK have been so far identified, PGK1 and PGK2. The isozymes have 87-88% identical amino acid sequence identity and though they are structurally and functionally similar, they have different localizations: PGK2, encoded by an autosomal gene, is unique to meiotic and postmeiotic spermatogenic cells, while PGK1, encoded on the X-chromosome, is ubiquitously expressed in all cells.