Palmetto Subdivision

The Palmetto Subdivision is a CSX Transportation rail line in the Tampa Bay region of Florida. It runs from East Tampa and roughly parallels U.S. Route 41 south through Ruskin to Palmetto and Bradenton. The Palmetto Subdivision ends just south of Tropicana Yard in Oneco, where it connects with the Seminole Gulf Railway, a shortline that continues south into Sarasota.

The line notably serves The Mosaic Company's Riverview phosphate plant near the Alafia River, TECO Energy's Big Bend Power Station in southern Hillsoborough County, and Port Manatee in northern Manatee County. It also serves the Tropicana Juice Plant in Bradenton, with Tropicana's Juice Train continuing to be the most consistent service on the line today.