Micrococcal nuclease
Micrococcal nuclease (EC 3.1.31.1, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T', staphylococcal nuclease, spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an endo-exonuclease that preferentially digests single-stranded nucleic acids. The rate of cleavage is 30 times greater at the 5' side of A or T than at G or C and results in the production of mononucleotides and oligonucleotides with terminal 3'-phosphates. The enzyme is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
| Micrococcal nuclease | |||||||||
|---|---|---|---|---|---|---|---|---|---|
Ribbon schematic of micrococcal nuclease 3D structure, with Ca2+ and TdtP inhibitor | |||||||||
| Identifiers | |||||||||
| EC no. | 3.1.31.1 | ||||||||
| CAS no. | 9013-53-0 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
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| Thermonuclease | |||||||
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| Identifiers | |||||||
| Organism | |||||||
| Symbol | nuc | ||||||
| UniProt | P00644 | ||||||
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| Staphylococcal nuclease | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | ? | ||||||||
| Pfam | PF00565 | ||||||||
| Pfam clan | CL0049 | ||||||||
| InterPro | IPR016071 | ||||||||
| PROSITE | PDOC00865 | ||||||||
| CATH | 1tt2 | ||||||||
| SCOP2 | 1tt2 / SCOPe / SUPFAM | ||||||||
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