ADA (buffer)

ADA is a zwitterionic organic chemical buffering agent; one of Good's buffers. It has a useful pH range of 6.0-7.2 in the physiological range, making it useful for cell culture work. It has a pKa of 6.6 with ΔpKa/°C of -0.011 and is most often prepared in 1 M NaOH where it has a solubility of 160 mg/mL.

ADA
Names
Systematic IUPAC name
2,2′-[(2-amino-2-oxoethyl)azanediyl]diacetic acid
Other names
ADA, N-(2-acetamido)iminodiacetic acid, N-(carbamoylmethyl)iminodiacetic acid
Identifiers
3D model (JSmol)
ECHA InfoCard 100.043.194
EC Number
  • 247-530-0
UNII
  • InChI=1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13)
    Key: QZTKDVCDBIDYMD-UHFFFAOYSA-N
  • C(C(=O)N)N(CC(=O)O)CC(=O)O
Properties
C6H10N2O5
Molar mass 190.155 g/mol
Acidity (pKa) 6.6
Hazards
Occupational safety and health (OHS/OSH):
Main hazards
 Irritant
GHS labelling:
Warning
H302, H315, H319, H335
P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
Flash point Non-flammable
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

ADA has been used in protein-free media for chicken embryo fibroblasts, as a chelating agent for H+, Ca2+, and Mg2+, and for isoelectric focusing in immobilized pH gradients. Its effects on dog kidney Na+/K+-ATPase and rat brain GABA receptors have also been studied. ADA does, however, alter coloring in bicinchoninic acid assays.

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