I am trying to calculate ka and ks, so I am using the test data available here: https://github.com/qiao-xin/Scripts_for_GB
Howerver,I have an error:
perl calculate_Ka_Ks_pipe.pl -d data/Ath.cds -g data/Ath.tandem.pairs -o result/Ath.td.kaks
Possible precedence issue with control flow operator at /home/dayana/miniconda3/envs/kaks/lib/site_perl/5.26.2/Bio/DB/IndexedBase.pm line 805.
ERROR: number of input seqs differ (aa: 0; nuc: 2)!!
aa '' nuc 'AT1G30410.1 AT1G30420.1' ERROR -- tmpdir-axt-converter-1194715-1690207616/c1.pep.aln -- File size is zero!!! at fa_prepareDATA4_kaks_calculator.pl line 190.
I would be happy if someone can help me.
Dayana.
I have no idea of the source of the problem.